Journal: Viruses

Article Title: Microarray-Based Detection of Antibodies against SARS-CoV-2 Proteins, Common Respiratory Viruses and Type I Interferons

doi: 10.3390/v13122553

Figure Lengend Snippet: Configuration of the microarray for detecting antibodies against respiratory viruses and type I IFNs. Abbreviations: SARS-CoV-2 S RBD—SARS-CoV-2 spike receptor-binding domain fragment Arg319-Phe541, SARS-CoV-2 N—SARS-CoV-2 nucleocapsid, Flu A—influenza A virus nucleoprotein, Flu B—influenza B virus nucleoprotein, HCoV OC43 S—OC43 coronavirus spike protein, HCoV HKU1 S—HKU1 coronavirus spike protein, IFN-ω—interferon ω, IFN-α—interferon α-2a, AdV—adenovirus, PIV-1—parainfluenza virus type 1, PIV-2—parainfluenza virus type 2, PIV-3—parainfluenza virus type 3, RSV—respiratory syncytial virus, SARS-CoV S—severe acute respiratory syndrome coronavirus spike protein, MERS CoV S—Middle East respiratory syndrome coronavirus spike protein.

Article Snippet: The following immobilized antigens were included in the microarray layout ( ): SARS-CoV-2 (spike RBD (S-RBD))—SARS-CoV-2 spike receptor-binding domain fragment Arg319-Phe541 (8COV1, HyTest, Turku, Finland), SARS-CoV-2 N—SARS-CoV-2 nucleocapsid (8COV3, HyTest, Finland), Flu-A—influenza A virus nucleoprotein (LS-G21636, LifeSpan BioSciences, Seattle, WA, USA), RSV—respiratory syncytial virus (8RSV79, HyTest, Finland), OC43 spike protein (DAGC131, Creative Diagnostics, New York, NY, USA), HKU1 spike protein (DAGC132, Creative Diagnostics, New York, NY, USA), IFN-ω—interferon ω (300-02J, PeproTech, Cranbury, NJ, USA); IFN-α-2a—interferon α-2a (11100-1, PBL Assay Science, Piscataway, NJ, USA), AdV—adenovirus (8AV13, HyTest, Finland), PIV-1—parainfluenza virus type 1 (8P76, HyTest, Finland), PIV-2—parainfluenza virus type 2 (8P76-2, HyTest, Finland), PIV-3—parainfluenza virus type 3 (8P76-3, HyTest, Finland), Flu-B—influenza B virus nucleoprotein (LS-G138331, LifeSpan BioSciences, Seattle, WA, USA), SARS-CoV (spike)—severe acute respiratory syndrome coronavirus spike protein (DAGC111, Creative Diagnostics, New York, NY, USA), and MERS-CoV (spike)—Middle East respiratory syndrome coronavirus spike protein (DAGH10300, Creative Diagnostics, New York, NY, USA).

Techniques: Microarray, Binding Assay

Journal: Scientific Reports

Article Title: Effects of Optical Turbulence and Density Gradients on Particle Image Velocimetry

doi: 10.1038/s41598-020-58077-5

Figure Lengend Snippet: Laboratory tank at NRLSSC. The schematic on the left illustrates the concept of PIV measurements (image source Dantec Dynamics, Inc.). The photo on the right shows the setup of the Particle Image Velocimetry (PIV) system for a measurement plane with a path length of about 1 m. The mirror is set up to direct the light sheet (green laser, wavelength 532 nm) into the tank at a 90 degree angle to illuminate a cross section. In the photo, the camera is looking on the bottom boundary, whereas for the experiments presented in this paper, the camera was positioned to look at the center height of the tank (after ).

Article Snippet: The PIV set up is a state-of-the-art system by Dantec Dynamics Inc. designed to measure two-dimensional cross-sectional planes of the convective flow in the SiTTE tank.

Techniques:

Journal: Scientific Reports

Article Title: Effects of Optical Turbulence and Density Gradients on Particle Image Velocimetry

doi: 10.1038/s41598-020-58077-5

Figure Lengend Snippet: Set up for experiments presented in this paper. The schematic on the left illustrates the concept of Laser Doppler Anemometer (LDA) measurements (image source Dantec Dynamics, Inc.). The photo on the right shows the setup of the LDA system at SiTTE to provide a reference measurement, as well as the PIV system shown in Fig. . The LDA system is positioned so that the intersection of the red laser beams (wavelength 660 nm) is in same plane as the PIV laser sheet.

Article Snippet: The PIV set up is a state-of-the-art system by Dantec Dynamics Inc. designed to measure two-dimensional cross-sectional planes of the convective flow in the SiTTE tank.

Techniques:

Journal: Cells

Article Title: PI 3-Kinase and the Histone Methyl-Transferase KMT2D Collaborate to Induce Arp2/3-Dependent Migration of Mammary Epithelial Cells

doi: 10.3390/cells13100876

Figure Lengend Snippet: PI 3-kinase activity is critical for collective cell migration. Confluent monolayers of parental, PIK3CA H1047R, KMT2D KO and double-mutant MCF10A cells are induced to migrate into the free space obtained by lifting a physical boundary. Displacement vectors within the monolayer are obtained using Particle Image Velocimetry (( A ), PIV). These vectors are analyzed over space and time for their position in terms of their magnitude (( B ), velocity) and directionality (( C ), order parameter, i.e., the cosine of the angle made by the displacement vector with respect to the vector oriented toward the free space). These parameters are represented as heat maps. There are three biological repeats with similar results, and four fields of view in each condition. Plots show the average of all measurements. Each pixel of the heatmaps averages all fields of view in all biological replicates over 42 µm and 40 min. These parameters are compared at a single time point or at a single distance from the edge using two-way ANOVA with Tukey’s test.

Article Snippet: Analysis of time-lapse images was performed using Particle Image Velocimetry (PIV) analysis with a MATLAB PIV script [ ].

Techniques: Activity Assay, Migration, Mutagenesis, Plasmid Preparation